GSM3263078: day05.Input.Batch2.Rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Pluripotent stem cell
Cell type
hESC derived cardiac cells
NA
NA
Attributes by original data submitter
Sample
source_name
ES derived cardiac mesoderm
tissue
ES derived cardiac mesoderm
differentiation time
day05
genotype
H9 MLC2v:H2B
matched input
N/A
chip antibody
none (input)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The ChIP-seq has been carried out as previously described (Jolma et al., 2013; Tuupanen et al., 2012; Yan et al., 2013). Briefly, 2 million cells were crosslinked with 1% formadehyde for 15 min at RT. The reaction was quenched by adding 125 mM of Glycine and incubating for 5 min at RT. Cells were lysed in RIPA buffer (10 mM TrisHCl pH 8.0, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% SDS, 0.1% sodium deoxycholate) supplemented with protease inhibitor (Roche). And chromatin was sonicated into short fragments (300-700 bp). The fragmented chromatin was incubated with antibodies to pull down the specific DNA bound TFs or histones. After intensive wash, DNA was purified and prepared as sequencing library using illumina Truseq LT kit. Several samples with different indexes were pooled together for 50 or 100 cycles of single read sequencing with illumina Hiseq 2500.