GSM3262277: LHX9-V5 ChIPseq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Epitope tags
Cell type
Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
colon
cell line
HCT116
tissue
colon
disease
colorectal carcinoma
genetic perturbation
Expressing LHX9-V5 open reading frame
chip antibody
V5 (Invitrogen #46-0705)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation was performed using SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling #9003). Monoclonal antibody against V5 tag was obtained from Invitrogen (V5 #46-0705) and used at 1:100 dilution Libraries were prepared by Molecular Biology Core Facilities at Dana Farber Cancer Institute according to Illumina's instruction