RNA extraction was performed using the RNeasy mini kit (Qiagen) following manufacturer's instruction. RNA samples were quantified and quality was evaluated using Bioanalyzer (RIN > 9.9). A total of 0.5 ug of RNA was used as starting material for library preparation with rRNA depletion using TruSeq Stranded Total RNA Library Prep kit (Ilumina) following the manufacturer´s instruction. Libraries were prepared according to Illumina instructions.