GSM3258586: ATAC-seq Act.5 h without CD28 rep2; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA
Attributes by original data submitter
Sample
source_name
ATAC-seq_Act.5 h_without_CD28
subject status
healthy donor
tissue
peripheral blood
cell type
primary naive CD4 T cells
activation
Act. 5 h with anti-CD3 antibody
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Nucleic DNA was processed with nextera transposome (illumina), and purified according to the protocol in Buenrostro et al (PMID: 24097267). Libraries were prepared according to the protocol in Buenrostro et al. Briefly, purified DNA was amplified with P5 and P7 primers in PCR, and 200-400 bp DNA was isolated by Ampure DNA purification beads.