Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ARID4B

Cell type

Cell type Class
Prostate
Cell type
PC-3
Primary Tissue
Prostate
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
PC3, ChIP fraction
cell line
PC3
cell type
metastatic prostate cancer cells
chip antibody
anti-ARID4B antibody (CAT#: A302-233A, LOT#: A302-233A-1,Bethyl Laboratories)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cell lysates were clarified from sonicated nuclei and the ARID4B-DNA complexes were isolated with the anti-ARID4B antibody (CAT#: A302-233A, LOT#: A302-233A-1, Bethyl Laboratories). Libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
34839290
Reads aligned (%)
71.2
Duplicates removed (%)
26.0
Number of peaks
4770 (qval < 1E-05)

hg38

Number of total reads
34839290
Reads aligned (%)
72.6
Duplicates removed (%)
25.0
Number of peaks
4832 (qval < 1E-05)

Base call quality data from DBCLS SRA