Antigen

Antigen Class

TFs and others

Antigen

ARID4B

Cell type

Cell type Class

Prostate

Cell type

PC-3

Primary Tissue

Prostate

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

PC3, ChIP fraction

cell line

PC3

cell type

metastatic prostate cancer cells

chip antibody

anti-ARID4B antibody (CAT#: A302-233A, LOT#: A302-233A-1,Bethyl Laboratories)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cell lysates were clarified from sonicated nuclei and the ARID4B-DNA complexes were isolated with the anti-ARID4B antibody (CAT#: A302-233A, LOT#: A302-233A-1, Bethyl Laboratories). Libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

34839290

Reads aligned (%)

96.8

Duplicates removed (%)

25.0

Number of peaks

4832 (qval < 1E-05)

hg19

Number of total reads

34839290

Reads aligned (%)

96.3

Duplicates removed (%)

26.0

Number of peaks

4770 (qval < 1E-05)