Sample information curated by ChIP-Atlas

Antigen

Antigen Class
ATAC-Seq
Antigen
ATAC-Seq

Cell type

Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA

Attributes by original data submitter

Sample

source_name
In-vitro stimulated CD69+ IL-2.eGFP– CD4 T cells
strain
C57BL/6 Il2eGFP
tissue
spleen/lymh nodes
genotype
IL-2.eGFP

Sequenced DNA Library

library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
cells sorted from activated CD4 T cells, washed with 1X PBS, and nuclei were isolated. The transposition reaction was carried out using Nextera DNA Sample Preparation Kit Illumina Nextera DNA Sample Preparation Kit

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
39243366
Reads aligned (%)
91.1
Duplicates removed (%)
21.6
Number of peaks
8371 (qval < 1E-05)

mm9

Number of total reads
39243366
Reads aligned (%)
91.0
Duplicates removed (%)
21.8
Number of peaks
8347 (qval < 1E-05)

Base call quality data from DBCLS SRA