Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
HDAC2

Cell type

Cell type Class
Muscle
Cell type
RH-4
NA
NA

Attributes by original data submitter

Sample

source_name
RH4
cell line
RH4
antibody
HDAC2, Abcam (cat7029 lotGR88809-14 pAb)
enrichment target
HDAC2
target function
NuRD Complex
treatment
untreated

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was extracted from ChIP-seq samples prepared following the Active Motif ChIP-IT High Sensitivity kit protocol. ATAC-seq DNA purification was performed by column (MiniElute PCR purification kit, Qiagen). Standard Illumina barcodes were introduced during library preparation

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
23541900
Reads aligned (%)
84.0
Duplicates removed (%)
13.3
Number of peaks
19100 (qval < 1E-05)

hg38

Number of total reads
23541900
Reads aligned (%)
85.2
Duplicates removed (%)
12.6
Number of peaks
19337 (qval < 1E-05)

Base call quality data from DBCLS SRA