ChIP-Atlas: SRX4312954

Antigen

Cell type Class: Gonad
Cell type: Spermatocytes
MeSH Description: Male germ cells derived from SPERMATOGONIA. The euploid primary spermatocytes undergo MEIOSIS and give rise to the haploid secondary spermatocytes which in turn give rise to SPERMATIDS.

library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: Spermatocytes were isolated and fixed by 1% paraformaldehyde solution. The chromatin was sheared to ~1000 bp by sonication and used for ChIP. For DMC1 library preparation, DNA was firstly end repaired by incubation with T4 DNA Ligase Reaction Buffer with dNTP, T4 DNA polymerase, Klenow Enzyme and T4 PNK, followed by addition of 3'-A overhangs using Klenow Fragment 3'-5' exo-. After denaturation of DNA , the adapters from TruSeq Nano DNA LD Library Prep Kit (set A, Illumina, FC-121-4001) were ligated with Quick Ligation kit. The libraries then were amplified using PCR Enhancer mix and primer cocktail (Illumina, FC-121-4001) for 12 cycles.