Antigen

Antigen Class

TFs and others

Antigen

RELA

Cell type

Cell type Class

Uterus

Cell type

HeLa

Primary Tissue

Cervix

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

HeLa

tissue

HeLa cells

genotype/variation

p65 KO, wt p65 rescue

tnf stimulation

0h TNF

chip antibody

p65 (C-20); Santa Cruz sc-372

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For RNA-Seq cells were harvested and total RNA was isolated using the NucleoSpin RNA Kit (Macherey-Nagel). For ChIP-Seq samples were crosslinked using Formaldehyde, Sonicated and chromatin was extracted using the p65 Antibody. DNA was purified NucleoSpin Gel and PCR Clean UP Kit with buffer NTB (Macherey-Nagel). RNA-Seq libraries were prepared from total RNA using the TruSeq Stranded total RNA LT kit (Illumina). ChIP-Seq libraries were prepared from purified ChIP DNA with the Microplex Library Preparation Kit (Diagenode)

Sequencing Platform

instrument_model

Illumina HiSeq 1500

hg38

Number of total reads

31743984

Reads aligned (%)

90.6

Duplicates removed (%)

67.9

Number of peaks

573 (qval < 1E-05)

hg19

Number of total reads

31743984

Reads aligned (%)

90.1

Duplicates removed (%)

69.2

Number of peaks

615 (qval < 1E-05)