Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Prostate

Cell type

LNCAP

Primary Tissue

Prostate

Tissue Diagnosis

Carcinoma

Sample

source_name

LNCaP (CaP)

treatment

Empty H3K27me3

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitation (ChIP) experiments were performed per standard protocols (Millipore, Billerica, MA) with minor modifications. Briefly, 2-5M cells were crosslinked with 1% formaldehyde for 10 min at 37 °C. This reaction was subsequently quenched with 0.125M glycine. All library prep and sequencing were 75bp single end on Illuminia Nextseq 500

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

29240202

Reads aligned (%)

86.9

Duplicates removed (%)

8.3

Number of peaks

778 (qval < 1E-05)

hg19

Number of total reads

29240202

Reads aligned (%)

86.5

Duplicates removed (%)

9.4

Number of peaks

680 (qval < 1E-05)