An encapsulated lymphatic organ through which venous blood filters.
Attributes by original data submitter
Sample
source_name
Tet2/3-WT_72h_ATAC
strain
C57BL/6
age
8-12 weeks
genotype/variation
Tet2/3-WT (Tet2-flox, Tet3-flox, Rosa26-LSL-YFP)
tissue
Spleen
molecule subtype
Tn5 (ATAC)
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
50,000 cells were collected by centrifugation and washed once with 50uL ice-cold PBS and centrifuged at 600 xg for 5 mins at 4°C. Cell pellets were resuspended in 50 uL of cold lysis buffer (10mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630) and spin down immediately at 500 xg for 10 mins at 4°C. Supernatant was discarded and nuclei were resuspended in 50uL transposition reaction mix (25uL 1x TD buffer fom Illuminia, 2.5 uL Tn5 transposase, 22.5 uL H2O), incubated at 37°C for 30 mins, and DNA was purified with a Qiagen MinElute kit (Qiagen). Library was amplified with KAPA HiFi HotStart Real-time PCR Master Mix (Roche) using indexed primers. ATAC-seq, 50bp paired-end with HiSeq2500