Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

Lymphoma, B-Cell

MeSH Description

A group of heterogeneous lymphoid tumors generally expressing one or more B-cell antigens or representing malignant transformations of B-lymphocytes.

Sample

source_name

primary patient samples from MCL, DHL and acute B-cell lymphoblastic lymphoma/leukemia (ALL) cells

chip antibody

H3K27AC (Abcam ab4729)

treatment

50nM THZ1

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Samples were homogenized with rotor-stator device. Lysates were sonicated via Bioruptor. Protein-DNA complexes were isolated via immunoprecipitation and prepared for sequencing with Rubicon Thruplex DNA-seq kit.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

22829192

Reads aligned (%)

96.8

Duplicates removed (%)

4.4

Number of peaks

15304 (qval < 1E-05)

hg19

Number of total reads

22829192

Reads aligned (%)

95.9

Duplicates removed (%)

5.4

Number of peaks

15191 (qval < 1E-05)