Antigen

Antigen Class

TFs and others

Antigen

Runx2

Cell type

Cell type Class

Bone

Cell type

MC3T3-E1

NA

NA

Sample

source_name

mineralizing MC3T3-E1 subclone 4 cells at day 28

cell type

mineralizing osteoblast

passages

28-31

strain

C57BL/6

chip antibody

Runx2 antibody (M70, Santa Cruz Biotechnologies, Inc.)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were obtained from sonicated nuclei and Runx2-DNA complexes were isolated with Runx2 antibody (M70). Libraries of purified DNA were generated using Illumina SR adapters (Illumina) following manufacturer’s manual, and were selected for the inserted fragments of 200 ± 50 bp, and sequenced 36 bases on an Illumina Genome Analyzer II.

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

mm10

Number of total reads

28418309

Reads aligned (%)

92.9

Duplicates removed (%)

7.7

Number of peaks

27987 (qval < 1E-05)

mm9

Number of total reads

28418309

Reads aligned (%)

92.8

Duplicates removed (%)

7.8

Number of peaks

27997 (qval < 1E-05)