Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Epidermis

Cell type

Melanocytes

MeSH Description

Mammalian pigment cells that produce MELANINS, pigments found mainly in the EPIDERMIS, but also in the eyes and the hair, by a process called melanogenesis. Coloration can be altered by the number of melanocytes or the amount of pigment produced and stored in the organelles called MELANOSOMES. The large non-mammalian melanin-containing cells are called MELANOPHORES.

Sample

source_name

Melanocyte

sirna

siMITF

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were prepared from sonicated cells and histone-DNA complexes were isolated with antibody. Illumina sequencing libraries were prepared from ChIP DNA using the NEBNext ChIP-seq library prep reagent set for Illumina (New England Biolabs) as per manufacturer's protocol with the following modifications: DNA purification with Qiaquick PCR purification or Minelute PCR purification kits, 2% agarose gel DNA size selection, custom indexed adapters, and 18 cycles of PCR amplification. The libraries were sequenced on an Illumina HiSeq 2000 (single-end, 75 bp).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

80016884

Reads aligned (%)

96.1

Duplicates removed (%)

76.2

Number of peaks

1360 (qval < 1E-05)

hg19

Number of total reads

80016884

Reads aligned (%)

95.2

Duplicates removed (%)

77.8

Number of peaks

1068 (qval < 1E-05)