Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EZH2

Cell type

Cell type Class
Blood
Cell type
Peripheral blood mononuclear cells
NA
NA

Attributes by original data submitter

Sample

source_name
Peripheral blood mononuclear cell
tumor type
Chronic lymphocytic leukemia
tumor classification
IGHV Mutated CLL
ngs seq
ChIP seq
chip antibody
EZH2 Ab

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP seq chromatin was extracted from CLL B cells and normal B cells, sonicated and specific antibody was used to pulldown the ChIP DNA. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303). Briefly, DNA was end-repaired using a combination of T4 DNA polymerase, E. coli DNA Pol I large fragment (Klenow polymerase) and T4 polynucleotide kinase. The blunt, phosphorylated ends were treated with Klenow fragment (32 to 52 exo minus) and dATP to yield a protruding 3- 'A' base for ligation of Illumina's adapters which have a single 'T' base overhang at the 3' end. After adapter ligation DNA was PCR amplified with Illumina primers for 15 cycles and library fragments of ~250 bp (insert plus adaptor and PCR primer sequences) were band isolated from an agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
24814674
Reads aligned (%)
175.6
Duplicates removed (%)
5.9
Number of peaks
5059 (qval < 1E-05)

hg38

Number of total reads
24814674
Reads aligned (%)
177.7
Duplicates removed (%)
5.8
Number of peaks
5065 (qval < 1E-05)

Base call quality data from DBCLS SRA