Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

Thymocytes

MeSH Description

HEMATOPOIETIC PROGENITOR CELLS that have migrated to the THYMUS where they differentiate into T-LYMPHOCYTES. Thymocytes are classified into maturational stages based on the expression of CELL SURFACE ANTIGENS.

Sample

source_name

Rag2-/- thymocyte

strain

129

sample type

Primary cell

cell type/line

thymocyte

age

4 weeks

genotype/variation

Rag2-/- thymocyte

antibody

H3K27ac (abcam ab4729)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nucleosomes were obtained by micrococcal nuclease digestion and antibody added. Pulldown was performed using protein A-agarose and bound DNA was purified by phenol-chloroform extraction Library preparation and high-throughput sequencing were performed by the Duke Center for Genomic and Computational Biology Core Facility

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

58102740

Reads aligned (%)

97.9

Duplicates removed (%)

26.0

Number of peaks

35183 (qval < 1E-05)

mm9

Number of total reads

58102740

Reads aligned (%)

97.9

Duplicates removed (%)

26.0

Number of peaks

35150 (qval < 1E-05)