Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Embryo
Cell type
Embryonic intestinal epithelial cells
NA
NA

Attributes by original data submitter

Sample

source_name
Mouse small intestinal epithelial cells
strain
CD1 wild type
cell type
Embryonic whole tissue from midgut
genotype
Wild-Type
developmental stage
E13

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
E13 small intestines were dissected and cross-linked for subsequent ChIP. Whole cell lysates were sonicated and incubated with 6 uL CDX2 antibody (A300-691A, Bethyl Labs.) overnight for immunoprecipitation. ThruPLEX DNA-seq Kit (Rubicon Genomics) was used to prepare libraries, and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
31046653
Reads aligned (%)
96.2
Duplicates removed (%)
13.2
Number of peaks
1862 (qval < 1E-05)

mm9

Number of total reads
31046653
Reads aligned (%)
96.0
Duplicates removed (%)
13.2
Number of peaks
1868 (qval < 1E-05)

Base call quality data from DBCLS SRA