GSM3181706: WT E12 Small Intestine ATACseq replicate2; Mus musculus; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Digestive tract
Cell type
Intestinal epithelium
NA
NA
Attributes by original data submitter
Sample
source_name
Mouse intestinal epithelial cells
strain
CD1 wild type
cell type
Embryonic epithelial cells from the small intestine
genotype
Wild-Type
developmental stage
E12
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
E12 small intestines were digested in 0.25% Trypsin EDTA at 37°C for 30 minutes followed by neutralization with FBS and Epcam + cells were sorted by flow cytometry. Cells were immediately used for chromatin transposition. Whole nuclei were used for chromatin transposition using Nextera Tn5 Transposase (Illumina) The transposed DNA was amplified using NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs), and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.