GSM3181680: Cdx2-/- (deleted at E15) Small Intestine ATACseq replicate1; Mus musculus; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Digestive tract
Cell type
Intestinal epithelium
NA
NA
Attributes by original data submitter
Sample
source_name
Mouse intestinal epithelial cells
strain
B6; 129 mixed background
cell type
Embryonic epithelial cells from the small intestine
genotype
Cdx2f/f; Villin-Cre
developmental stage
E18
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Villi from E18 intestines were isolated by incubation in 5mM EDTA in PBS for 45 minutes at 4°C. Single cells were dissociated with 4X TrypLE in DMEM for 45 minutes at 37°C. Cells were immediately used for chromatin transposition. Whole nuclei were used for chromatin transposition using Nextera Tn5 Transposase (Illumina) The transposed DNA was amplified using NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs), and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.