GSM3181678: WT P1 Small Intestine ATACseq replicate2; Mus musculus; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Digestive tract
Cell type
Intestinal epithelium
NA
NA
Attributes by original data submitter
Sample
source_name
Mouse intestinal epithelial cells
strain
CD1 wild type
cell type
Post Embryonic Epithelial cells from the intestine
genotype
Wild-Type
developmental stage
P1
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Villi from P1 intestines were isolated by incubation in 5mM EDTA in PBS for 45 minutes at 4°C. Single cells were dissociated with 4X TrypLE in DMEM for 45 minutes at 37°C. Cells were immediately used for chromatin transposition. Whole nuclei were used for chromatin transposition using Nextera Tn5 Transposase (Illumina) The transposed DNA was amplified using NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs), and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.