GSM3178255: R1 WT ES H3K27ac; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
Embryonic stem cell
cell line
R1
cell type
Embryonic stem cell
genotype
WT
state
ES
chip antibody
H3K27ac (Active Motif, 39133, lot 31917009)
young_id
20180207_6804
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked on plates with 1% formaldehyde in media for 15 minutes at room temperature. 50 million cells were used per ChIP reaction. Whole cell extracts were sonicated on a QSonica Q700 tip sonicator to solubilize the chromatin. The chromatin extracts containing DNA fragments with an average size of 500 bp were immunoprecipitated using different antibodies. Library construction was performed by the Whitehead Institute Genome Technology Core using the Swift Biosciences Accel-NGS 2S Plus DNA Library Kit according to manufacturer's protocol.