Antigen

Antigen Class

Histone

Antigen

H3K9me3

Cell type

Cell type Class

Neural

Cell type

Neurons

NA

NA

Sample

source_name

neuron cells

treatment

induced from hNPC which is induced from hIPS

chip antibody

H3K9me3 (Abcam,ab8898)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For Hi-C, nuclei were extraced after fixing using a cell lysis buffer. For ChIP-seq, nuclei were extracted and chromatins were fragmented by sonication. The TF/histone-DNA complexes were isolated by antibody. All Hi-C libraries were constructed following illumina insctructions accompanying Truseq sample preparation kit. Random indexes were introduced for eHi-C libraries to remove PCR duplication. Generally, PCR amplification was done with 7-9 cycles. All ChIP-seq libraries were generated following a ChIPmentation protocol with Nextera adapters.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

15474445

Reads aligned (%)

94.8

Duplicates removed (%)

29.4

Number of peaks

384 (qval < 1E-05)

hg19

Number of total reads

15474445

Reads aligned (%)

91.1

Duplicates removed (%)

32.7

Number of peaks

308 (qval < 1E-05)