ChIP-Atlas: SRX4168193

Antigen

Cell type Class: Blood
Cell type: Macrophages
MeSH Description: The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

source_name: Primary mouse bone marrow derived macrophages (BMDM)
strain/background: C57BL/6
cell type: Primary mouse bone marrow derived macrophages (BMDM)
treatment: none
time after lps/ifng treatment: 0h
chip antibody: H3K18ac (Abcam, ab1191, lot GR 300534-1)
spike-in information: spike-in chromatin (Active motif, Catalog No. 53083), spike-in antibody (Active motif, Catalog No. 61686)

library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: Chromatin was digested with MNase, enriched with indicated antibodies, and purified with QIAquick PCR purification kit (Qiagen, #28106). Total RNAs were prepared by following protocols from RNeasy Plus Mini Kit (Qiagen, 74134). ChIP-seq libraries were constructed with Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences) according to the manufacturer's protocol. The libraries were then amplified and assessed for fragment size using TapeStation (Agilent) and quantified using Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific). RNA-seq libraries were constructed with Illumina TruSeq Stranded mRNA Library Prep Kit Set A (Illumina, RS- 122-2101). The libraries' size was selected by using the Agencourt AMPure XP beads (Beckman Coulter, A63882), with average size 400bps.