Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC derived endodermal cells
NA
NA

Attributes by original data submitter

Sample

source_name
human Naïve endoderm + induced CDX2 culture cells
cell type
human Naive endoderm + induced CDX2 culture cells
genotype
Wild-Type
treatment
Doxycycline

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Naïve endoderm + induced CDX2 culture cells were fixed and frozen for subsequent chromatin pulldown Whole cell lysates were sonicated and incubated with 6 uL CDX2 antibody overnight for immunoprecipitation. The ThruPLEX DNA-seq Kit (Rubicon Genomics) was used to prepare libraries

Sequencing Platform

instrument_model
NextSeq 550

hg38

Number of total reads
41485106
Reads aligned (%)
99.3
Duplicates removed (%)
7.2
Number of peaks
131853 (qval < 1E-05)

hg19

Number of total reads
41485106
Reads aligned (%)
98.7
Duplicates removed (%)
7.5
Number of peaks
131708 (qval < 1E-05)

Base call quality data from DBCLS SRA