Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Digestive tract
Cell type
Intestinal culture cells
NA
NA

Attributes by original data submitter

Sample

source_name
human Wnt+Fgf-specified intestinal culture cells
cell type
human Wnt+Fgf-specified intestinal culture cells
genotype
Wild-Type
treatment
NO

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Intestinal Specified Endoderm culture cells were fixed and frozen for subsequent chromatin pulldown Whole cell lysates were sonicated and incubated with 6 uL CDX2 antibody overnight for immunoprecipitation. The ThruPLEX DNA-seq Kit (Rubicon Genomics) was used to prepare libraries

Sequencing Platform

instrument_model
NextSeq 550

hg38

Number of total reads
51898193
Reads aligned (%)
98.8
Duplicates removed (%)
6.8
Number of peaks
67298 (qval < 1E-05)

hg19

Number of total reads
51898193
Reads aligned (%)
97.5
Duplicates removed (%)
7.1
Number of peaks
70289 (qval < 1E-05)

Base call quality data from DBCLS SRA