Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Embryo
Cell type
Midgut
NA
NA

Attributes by original data submitter

Sample

source_name
Mouse midgut cells
developmental stage
E16.5
strain
C57BL6; mixed background
cell type
Embryonic whole tissue from midgut
genotype
Wild-Type(Cdx2 f/f; vil-Cre-ERT2 X Cdx2 f/f; vil-Cre-ERT2)
treatment
NO

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
midguts were dissected and fixed for subsequent chromatin pull down. Whole cell lysates were sonicated and incubated with 6 uL CDX2 antibody overnight for immunoprecipitation. The ThruPLEX DNA-seq Kit (Rubicon Genomics) was used to prepare libraries

Sequencing Platform

instrument_model
NextSeq 550

mm10

Number of total reads
21390466
Reads aligned (%)
90.8
Duplicates removed (%)
17.9
Number of peaks
997 (qval < 1E-05)

mm9

Number of total reads
21390466
Reads aligned (%)
90.6
Duplicates removed (%)
18.0
Number of peaks
761 (qval < 1E-05)

Base call quality data from DBCLS SRA