Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Embryo

Cell type

Midgut

NA

NA

Sample

source_name

Mouse midgut cells

developmental stage

E13.5

strain

CD1 wild type

cell type

Embryonic whole tissue from midgut

genotype

Wild-Type

treatment

NO

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

midguts were dissected and fixed for subsequent chromatin pull down. Whole cell lysates were sonicated and incubated with 6 uL CDX2 antibody overnight for immunoprecipitation. The ThruPLEX DNA-seq Kit (Rubicon Genomics) was used to prepare libraries

Sequencing Platform

instrument_model

NextSeq 550

mm10

Number of total reads

31046653

Reads aligned (%)

96.2

Duplicates removed (%)

13.2

Number of peaks

1801 (qval < 1E-05)

mm9

Number of total reads

31046653

Reads aligned (%)

96.0

Duplicates removed (%)

13.2

Number of peaks

1802 (qval < 1E-05)