Antigen

Antigen Class

TFs and others

Antigen

Epitope tags

Cell type

Cell type Class

Pluripotent stem cell

Cell type

F9

Tissue

Testis

Disease

Embryonal Carcinoma; Testicular Teratoma

Sample

source_name

F9 EC cells

cell type

F9 EC cells

overexpression

Zkscan17

chip antibody

HA (ab9110)

tag

N-3XHA

ChIP

O'Geen et al. 2010

mapping

Bowtie (-5 3 --best)

peak calling parameters

p-value < 1e-5, enrichment > 10

input

F9_Input + HA_control

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was purified and immunoprecipitated according to previously described protocols as indicated in the sample information. DNA SMART kit (Takara) or Truseq (Illumina)

Sequencing Platform

instrument_model

Illumina HiSeq 3000

mm10

Number of total reads

15216360

Reads aligned (%)

90.7

Duplicates removed (%)

9.9

Number of peaks

220 (qval < 1E-05)

mm9

Number of total reads

15216360

Reads aligned (%)

90.4

Duplicates removed (%)

9.8

Number of peaks

241 (qval < 1E-05)