Antigen

Antigen Class

TFs and others

Antigen

Epitope tags

Cell type

Cell type Class

Pluripotent stem cell

Cell type

F9

Tissue

Testis

Disease

Embryonal Carcinoma; Testicular Teratoma

Sample

source_name

F9 EC cells

cell type

F9 EC cells

overexpression

Gm30910

chip antibody

FLAG (F1804)

tag

N-3XFLAG

ChIP

O'Geen et al. 2010

mapping

Bowtie (-5 3 --best)

peak calling parameters

p-value < 1e-10, enrichment > 20

input

F9_Input + FLAG_control

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was purified and immunoprecipitated according to previously described protocols as indicated in the sample information. DNA SMART kit (Takara) or Truseq (Illumina)

Sequencing Platform

instrument_model

Illumina HiSeq 3000

mm10

Number of total reads

23622945

Reads aligned (%)

88.8

Duplicates removed (%)

12.1

Number of peaks

508 (qval < 1E-05)

mm9

Number of total reads

23622945

Reads aligned (%)

88.6

Duplicates removed (%)

12.1

Number of peaks

544 (qval < 1E-05)