GSM3173673: H3K4me3 Chr4-cl KO B6/129 ES cells #6; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me3
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
B6/129 ES cells
genotype/variation
Chr4-cl KO
strain
B6/129
cell type
ES cells
culture media
20% FCS
chip antibody
H3K4me3 (ab8580)
ChIP
ChIP (O'Geen et al. 2010)
mapping
Bowtie (-5 3 --best)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Native chromatin was purified and immunoprecipitated as described previously (Karimi et al., 2011). Cross-linked ChIP was performed as described previously (references are given in the sample information). Illumina Truseq (Chr2-cl WT and KO ES cells) or DNA SMART kit (Takara) (all other samples)