GSM3173660: H3K9me3 Chr2-cl KO B6 ES cells #G11; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K9me3
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
B6 ES cells
genotype/variation
Chr4-cl KO
strain
B6
cell type
ES cells
culture media
2i
chip antibody
H3K9me3 (Diagenode, C15410056)
ChIP
ChIP (Ecco et al. 2016)
mapping
Bowtie2
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Native chromatin was purified and immunoprecipitated as described previously (Karimi et al., 2011). Cross-linked ChIP was performed as described previously (references are given in the sample information). Illumina Truseq (Chr2-cl WT and KO ES cells) or DNA SMART kit (Takara) (all other samples)