Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Blood

Cell type

Th2 Cells

MeSH Description

A subset of helper-inducer T-lymphocytes which synthesize and secrete the INTERLEUKINS IL-4; IL-5; IL-6; and IL-10. These cytokines influence B-cell development and antibody production as well as augmenting humoral responses.

Sample

source_name

naive CD4 T cells

strain background

C57BL/6

genotype/variation

Menin wild type

tissue

spleen

cell type

splenic naive CD4 T cells

chip antibody

H3K4me3

chip antibody vendor

Active Motif

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and transcription factor- or histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the ChIP Sample Prep Kit

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

mm10

Number of total reads

33937709

Reads aligned (%)

93.8

Duplicates removed (%)

27.9

Number of peaks

20803 (qval < 1E-05)

mm9

Number of total reads

33937709

Reads aligned (%)

93.6

Duplicates removed (%)

27.9

Number of peaks

20823 (qval < 1E-05)