GSM3165675: H1048 sgHROSA H3K27ac CHIP; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Lung
Cell type
NCI-H1048
Primary Tissue
Lung
Site of Extraction
Effusion, Pleural
Tissue Diagnosis
Carcinoma Small Cell
Attributes by original data submitter
Sample
source_name
Small cell lung cancer
cell line
NCI-H1048-cas9
cell type
Small cell lung cancer
treatment
infected with sgNeg, collected cells at day 2 post infection
chip antibody
H3K27ac (abcam ab4729)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cell lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using Illumina TruSeq ChIP Sample Prep kit following manufacture's protocol. Briefly, ChIP DNA was end-repaired, 3'-adenylated, and then ligated with indexed adaptor. These DNA was size selected (200-400 bp) via agarose gel electrophoresis, and PCR amplified. Final products were purified using AMPure XP beads.