GSM3165625: DMS79 2 H3K27ac; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Lung
Cell type
DMS 79
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma Small Cell
Attributes by original data submitter
Sample
source_name
Small cell lung cancer
cell line
DMS79
cell type
Small cell lung cancer
treatment
exponentially growing cells with no infection
chip antibody
H3K27ac (abcam ab4729)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cell lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using Illumina TruSeq ChIP Sample Prep kit following manufacture's protocol. Briefly, ChIP DNA was end-repaired, 3'-adenylated, and then ligated with indexed adaptor. These DNA was size selected (200-400 bp) via agarose gel electrophoresis, and PCR amplified. Final products were purified using AMPure XP beads.