GSM3163862: input DNA rrep2 3hr Input; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Pluripotent stem cell
Cell type
hESC derived neural cells
NA
NA
Attributes by original data submitter
Sample
source_name
Neural progenitor cells
cell type
H1 hESC-derived neural progenitor cells
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked with 1% formaldehyde. Chromatin was shared with Covaris S2 sonicator. ChIP was performed using LowCell# ChIP kit (Diagenode). H3K27ac (Abcam, ab4729) and H3K27me3 antibodies (EMD Millipore, 07-449) were used. Library construction was performed using ThruPLEX DNA-seq kit (Rubicon Genomics) accoring to manufacture's protocol.