Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
FOXP1

Cell type

Cell type Class
Blood
Cell type
B cells
NA
NA

Attributes by original data submitter

Sample

source_name
Naïve IgD+ B cells
tissue
Primary tonsilar B cells
chip antibody
FOXP1 (ab4729, Rabbit, 2 ug)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was sheared to 200-400bp, incubated with H3K27ac antibody (abcam 4729) overnight at 4C on a rocker, antibody chromatin complex was captured by ProteinA agarose beads, washed with low salt, high salt, LiCl, and TE buffer, eluted and then de-crosslinked at 65C with 0.3M NaCl final for at least 6 hr. Final chormatin was purified using Qiagen PCR purification columns. Libraries were prepared using the Illumina Truseq ChIPseq Library preparation Kit following the manufacture's instructions (Illumina, CA).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
199652020
Reads aligned (%)
51.5
Duplicates removed (%)
44.9
Number of peaks
16991 (qval < 1E-05)

hg38

Number of total reads
199652020
Reads aligned (%)
53.6
Duplicates removed (%)
43.2
Number of peaks
18189 (qval < 1E-05)

Base call quality data from DBCLS SRA