10^7 cells per condition were cross-linked in 1% formaldehyde, lysed and sonicated as described (Tzelepis et al., 2016). Chromatin was immunoprecipitated with an anti-H3K9ac antibody (ab4441, AbCam) and captured with 1:1 protein A and protein G magnetic beads. After reverse cross-linking, immunoprecipitated and input DNA were purified using a Qiagen PCR purification kit. Standard Illumina ChIP library protocols with Nextera indices.