GSM3150000: 1700 PC neg [ATAC-Seq]; Mus musculus; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Blood
Cell type
Plasmablasts
NA
NA
Attributes by original data submitter
Sample
source_name
spleen
cell type
Plasmablasts
gender
female
sorting markers
Thy1.1- F4/80- CD11c- CD138+
strain
C57BL/6J
tissue
spleen
stimulation
LPS
lsd1 status
wild-type
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Total RNA was extracted using the Zymo Quick-RNA MicroPrep Kit 10,000 cells were centrifuged for 10 minutes at 500xg, resuspended in 50 ul of Lysis Buffer (contents), and immediately centrifuged for 30 minutes at 500xg. Tagmentation was performed by resuspending cells in 25 ul of Tagmentation mix (1x TD Buffer, 2.5 ul Tn5 Transpoase) and incubating for 60 minutes at 37°C. DNA was purified and amplified into a sequencing library with KAPA HiFi Polymerase and the Nextera Indexing Kit Primers (Illumina, Inc).