Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Embryo

Cell type

Mixed embryo

NA

NA

Sample

source_name

Mixed Embryos

tissue

whole larvae

strain

N2

Stage

MxE

chip antibody

anti-H3K4me3

chip antibody supplier

Abcam

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitations and library preparations were conducted as in (McMurchy et al., 2017), using formaldehyde as a fixitive for the H3K4me3 ChIPs (30ug DNA, 2.5ug antibody) and formaldehyde and EGS as fixatives for the SIN-3 (15ug DNA, 2.5ug antibody) and HDA-1 (30ug DNA, 2.5ug antibody) ChIPs. Approximately 10% C. briggsae chromatin extract was spiked into the C. elegans extract for the H3K4me3 ChIPs and 5% into the HDA-1 ChIPs. The HDA-1 antibody did not detect C. briggsae HDA-1 and so was not used for normalization.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

ce11

Number of total reads

13293366

Reads aligned (%)

84.8

Duplicates removed (%)

36.1

Number of peaks

6862 (qval < 1E-05)

ce10

Number of total reads

13293366

Reads aligned (%)

84.8

Duplicates removed (%)

36.1

Number of peaks

6865 (qval < 1E-05)