Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K36me3

Cell type

Cell type Class
Liver
Cell type
Liver
MeSH Description
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Attributes by original data submitter

Sample

source_name
Liver
strain
C57BL/6
experiment
ChIP-Seq
chip antibody
anti-H3K36me3 Abcam Cat N. ab9050 batch 927884
time point
0 h

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq was performed with the 'MicroPlex Library Preparation' kit, from Diagenode 10 ng of immunoprecipitated material was used for sequencing library preparation. Total input was also prepared for sequencing at each time point and replicate. Paired-end sequencing libraries were prepared with the 'MicroPlex Library Preparation' kit, from Diagenode, following the instructions of the manufacturer (Diagenode, catalog no C05010011). No size selection was performed and the chromatin was amplified with 14 cycles of PCR. 100 nucleotides at the fragment ends were sequenced with paired-end sequencing technology from HiSeq 2500 (Illumina).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
54085684
Reads aligned (%)
0.0
Duplicates removed (%)
5.4
Number of peaks
23908 (qval < 1E-05)

mm9

Number of total reads
54085684
Reads aligned (%)
0.0
Duplicates removed (%)
5.9
Number of peaks
23917 (qval < 1E-05)

Base call quality data from DBCLS SRA