Sample information curated by ChIP-Atlas

Antigen

Antigen Class
ATAC-Seq
Antigen
ATAC-Seq

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC derived keratinocyte
NA
NA

Attributes by original data submitter

Sample

source_name
mature ketatinocyte from hESC-epidermal differentiation
originating cell line
H9
cell type
mature keratinocyte differentiated from hESC
treatment and stage
mature keratinocyte from terminal differentiation

Sequenced DNA Library

library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Around 50,000 cells were used for each transposition reaction. Nuclei were prepared prior to transposition. Sequencing libraries were constructed using a modified version of the Illumina Nextera DNA Sample prep kit.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
74854971
Reads aligned (%)
93.0
Duplicates removed (%)
50.4
Number of peaks
10237 (qval < 1E-05)

hg19

Number of total reads
74854971
Reads aligned (%)
92.5
Duplicates removed (%)
50.8
Number of peaks
10214 (qval < 1E-05)

Base call quality data from DBCLS SRA