Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Embryo

Cell type

Embryonic limb

NA

NA

Sample

source_name

embryonic mouse limb buds

tissues

embryonic mouse limb buds

chip-seq antibody

H3K4me3 (ab8580,Abcam)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Mouse embryonic limb buds lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibodies ChIP-seq DNA libraries wereprepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

48869392

Reads aligned (%)

98.2

Duplicates removed (%)

40.6

Number of peaks

22298 (qval < 1E-05)

mm9

Number of total reads

48869392

Reads aligned (%)

97.8

Duplicates removed (%)

40.6

Number of peaks

22324 (qval < 1E-05)