Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

Liver

background strain

C57BL/6

tissue

Liver

chip antibody

H3K27ac (Abcam, ab4729)

age

16-week old

genotype

WT

Sex

female

diet

HFD

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Fresh mouse livers were crosslinked with 2 mM disuccinimidyl glutarate (DSG) for 30 min, followed by 1% formaldehyde for 10 min at room temperature. Nuclei were isolated, sonicated and incubated with magnetic bead-antibody complexes.  Briefly, DNA was end-repaired and ligated to stem-loop adaptors. Then DNA was PCR amplified with Illumina-compatible primers for 9 cycles and library fragments of 200 to 400 bp were selected using Ampure XP beads.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

22968993

Reads aligned (%)

98.7

Duplicates removed (%)

4.3

Number of peaks

13481 (qval < 1E-05)

mm9

Number of total reads

22968993

Reads aligned (%)

98.6

Duplicates removed (%)

4.4

Number of peaks

13516 (qval < 1E-05)