GSM3132738: ATAC-seq DE-WT2; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Pluripotent stem cell
Cell type
Differentiated hESCs
NA
NA
Attributes by original data submitter
Sample
source_name
ES-derived progenitors
tissue
ES-derived progenitors
differentiation stage
Definitive Endoderm
genotype
wild type
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
50,000 cells were lysed in ATAC lysis buffer and tagmented (Illumina Nextera DNA Library Preparation Kit). After adding SDS, the samples were purified with 2X Agencourt AMPure XP beads (Beckman Coulter A63881) Samples were amplified using NEBNext Q5 Hot Start HiFi PCR Master Mix. The concentration of samples was checked using PicoGreen and median fragment size was checked using the Agilent D1000 screentape on the Agilent Technologies 2200 TapeStation. Samples were sequenced PE50 on a HiSeq 2500.