Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Muscle

Cell type

C2C12

Primary Tissue

Skeletal Muscle

Tissue Diagnosis

NOS

Sample

source_name

C2C12 cells

tissue

C2C12 cells

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

RNA was extracted using Trizol, as per the manufacturer's instructions. Histone-DNA complexes for ChIP-Seq were recovered by immunoprecipitation using cell lysates from clarified sonicated nuclei. Libraries were constructed as per the manufacturers instructions. For RNASeq: 500 ng RNA was used with the Illumina stranded total RNASeq protocol; whereas, for ChIP-Seq the Illumina Nextera protocol was used.

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

15947859

Reads aligned (%)

84.5

Duplicates removed (%)

43.3

Number of peaks

340 (qval < 1E-05)

mm9

Number of total reads

15947859

Reads aligned (%)

83.8

Duplicates removed (%)

43.2

Number of peaks

379 (qval < 1E-05)