GSM3132571: DM HYP A H3K27me3 ChIPSeq; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Muscle
Cell type
C2C12
Primary Tissue
Skeletal Muscle
Tissue Diagnosis
NOS
Attributes by original data submitter
Sample
source_name
C2C12 cells
tissue
C2C12 cells
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA was extracted using Trizol, as per the manufacturer's instructions. Histone-DNA complexes for ChIP-Seq were recovered by immunoprecipitation using cell lysates from clarified sonicated nuclei. Libraries were constructed as per the manufacturers instructions. For RNASeq: 500 ng RNA was used with the Illumina stranded total RNASeq protocol; whereas, for ChIP-Seq the Illumina Nextera protocol was used.