GSM1300607: DNase mES Day6 Prepancreatic Endoderm; Mus musculus; DNase-Hypersensitivity
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
DNase-seq
Antigen
DNase-Seq
Cell type
Cell type Class
Pluripotent stem cell
Cell type
mESC derived pancreatic cells
NA
NA
Attributes by original data submitter
Sample
source_name
Murine Prepancreatic Endoderm Cells
cell type
mESC-derived Prepancretic Endoderm Cells
strain
129P2/OlaHsd
datatype
DNase-seq
cell sex
F
Sequenced DNA Library
library_strategy
DNase-Hypersensitivity
library_source
GENOMIC
library_selection
DNase
library_construction_protocol
Samples prepared into Illumina libraries using the Beckman Coulter Genomics SPRI-works system using custom adapters. 6nt 3’ barcodes were added during PCR enrichment and the resulting fragments were evaluated using Agilent BioAnalyzer 2100. Samples were sequenced one-per-lane using an Illumina HiSeq 2000. Libraries were diluted to 10nM and then run on qPCR with p5 and p7 primers to determine final flow cell loading concentration, normalized to a PhiX control library. PhiX spike: Each lane was spiked with approximately 0.25-0.5% PhiX control library. Samples were run one-per-lane using Standard Illumina protocols using the following: TruSeq SR/PE Cluster kit V3 cBot, TruSeq SBS Reagent Kit V3, RTA version 1.12.4.2, and Control software version 1.4.8.