Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Unclassified

Cell type

Unclassified

NA

NA

Sample

source_name

human secondary fibroblasts

cell type

hiF-T cells carrying DOX-inducible, polycistronic human OCT4/KLF4/c-MYC/SOX2 (OKMS) cassette

tissue

human secondary fibroblasts

passages/stage

13-18

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Approximately 5 million cells were used per IP reaction. The cells were fixed using 1.1% formaldehyde. iDeal ChIP-seq Kit for Transcription Factors (Diagenode) was used for chromatin shearing and all the subsequent steps of ChIP-seq library preparation Library preparation was done following standard Bioo Scientific protocol and NEXTflex® qRNA-Seq™ Kit v2 (Bioo Scientific). Libraries were sequnced using single end sequencing length of a 75 nucleotides on a HiSeq400 machine (Illumina).

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

38173990

Reads aligned (%)

95.7

Duplicates removed (%)

3.5

Number of peaks

822 (qval < 1E-05)

hg19

Number of total reads

38173990

Reads aligned (%)

93.5

Duplicates removed (%)

3.6

Number of peaks

359 (qval < 1E-05)