Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

Monocytes

MeSH Description

Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.

Sample

source_name

Monocytes

genotype

wildtype

cell markers

Ter119- B220- CD3- NK1.1- CD115+ cKit- CD34- FcgRII/III+ Ly6G- CD11b+

tissue

bone marrow

chip-antibody

ab4729, Abcam

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Genomic DNA extraction protocol not provided. Precipitated DNA were mixed with 0-2 ng E. Coli DNA, depending on the amount of ChIP DNA isolated. This mix was amplified using NEB Next ChIP-seq sample prep reagent set 1 (New England Biolabs) according to manufacturer's protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

86663476

Reads aligned (%)

86.9

Duplicates removed (%)

40.9

Number of peaks

10848 (qval < 1E-05)

mm9

Number of total reads

86663476

Reads aligned (%)

86.7

Duplicates removed (%)

40.9

Number of peaks

10895 (qval < 1E-05)