Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
E14 mESC-derived EB
cell type
E14 mESC-derived EB
status
Differentiated at week2
genotype
WT
antibody
anti-H3K27me3 (07-499, Millipore)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Histone-DNA and TF-DNA complexes were isolated with antibody Illumina TrueSeq ChIP Sample Preparation Kit

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
12157977
Reads aligned (%)
96.1
Duplicates removed (%)
19.0
Number of peaks
164 (qval < 1E-05)

mm9

Number of total reads
12157977
Reads aligned (%)
95.8
Duplicates removed (%)
19.1
Number of peaks
184 (qval < 1E-05)

Base call quality data from DBCLS SRA